To eliminate those non-cellulolytic contaminants, serial dilution and parallel streaking on the cellulose medium was repeatedly performed. ( 2008) also confirmed that the cellulose-degrading culture contains four organisms, but only one can hydrolyze cellulose, and the others are not directly linked to cellulose hydrolysis. ![]() In our study of cellulose degradation, it was found that the cellulolytic Cytophagales were always associated with some non-cellulolytic microbes in their process of cellulose digestion. Thus, search for the potential source of cellulolytic activity is continuing in the interest of successful bioconversion of lignocellulosic biomass. However, the ability of major cellulolytic member of microbial strains identified so far produced a limiting level of one or other enzymatic component (Maki et al. Both Trichoderma reesi and Aspergillus niger were co-cultured to increase the levels of different enzymatic components (Kumar et al. ![]() Synergistic cooperation of different enzymes is a prerequisite for the efficient degradation of cellulose (Jalak et al. Most known cellulolytic organisms produce multiple cellulases that act synergistically on native cellulose (Wilson 2008) as well as produce some other proteins that enhance cellulose hydrolysis (Wang et al. Therefore, efficient degradation is the result of multiple activities working synergistically to efficiently solubilize crystalline cellulose (Sánchez et al. Cellulose consists of only a linear structure of β-1,4-linked glucose residues, but is very difficult to be degraded, because it is highly crystalline and often tightly intertwined together with hemicelluloses and other polymers presenting a barrier to degradation by cellulase (Carpita and Gibeaut 1993).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |